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- References [1]
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- Western blot [2]
- Immunohistochemistry [7]
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- Product number
- NBP1-91657 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-91657, RRID:AB_11030810
- Product name
- Rabbit Polyclonal ALDH1A3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse ALDH1A3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A Pan-ALDH1A Inhibitor Induces Necroptosis in Ovarian Cancer Stem-like Cells.
Chefetz I, Grimley E, Yang K, Hong L, Vinogradova EV, Suciu R, Kovalenko I, Karnak D, Morgan CA, Chtcherbinine M, Buchman C, Huddle B, Barraza S, Morgan M, Bernstein KA, Yoon E, Lombard DB, Bild A, Mehta G, Romero I, Chiang CY, Landen C, Cravatt B, Hurley TD, Larsen SD, Buckanovich RJ
Cell reports 2019 Mar 12;26(11):3061-3075.e6
Cell reports 2019 Mar 12;26(11):3061-3075.e6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH1A3 Antibody [NBP1-91657] - Analysis in human cell line PC-3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALDH1A3 Antibody [NBP1-91657] - Analysis in human cell lines A-431 and Caco-2 using anti-ALDH1A3 antibody. Corresponding ALDH1A3 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP1-91657] - Staining of human prostate shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP1-91657] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: ALDH1A3 Antibody [NBP1-91657] - Aldh1a3 expression on E11.5 mouse embryo eye. Heat induced antigen retrieval at pH 6.0 was done for 20 minutes with mouse E11.5 coronal cryosection. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP1-91657] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP1-91657] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ALDH1A3 Antibody [NBP1-91657] - Staining of human skeletal muscle shows no positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALDH1A3 Antibody [NBP1-91657] - Analysis in human prostate and skeletal muscle tissues. Corresponding ALDH1A3 RNA-seq data are presented for the same tissues.
