MA5-14934

antibody from Invitrogen Antibodies

Targeting: KDM1A AOF2, BHC110, KDM1, KIAA0601, LSD1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14934

Provider

Invitrogen Antibodies

Product name

LSD1 Monoclonal Antibody (K.884.2)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

K.884.2

Vial size

100 μL

Concentration

17 μg/mL

Storage

-20°C

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of LSD1 was performed using 70 % confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with LSD1 Monoclonal Antibody (K.884.2) (Product # MA5-14934) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of LSD1 in HeLa cells using a LSD1 monoclonal antibody (Product # MA5-14934) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of LSD1 in HeLa cells using a LSD1 monoclonal antibody (Product # MA5-14934) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LSD1 in paraffin-embedded human lung carcinoma using a LSD1 monoclonal antibody (Product # MA5-14934).

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) assay of endogenous LSD1 protein using LSD1 Antibody: ChIP was performed using LSD1 Polyclonal Antibody (Product # MA5-14934, 5 µg) on sheared chromatin from A549 and A549 cells treated with Dexamethasone (100 nM, 2hours) using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to BIRC3, CDKN1C, DUSP1 (active) and MYOD, SAT alpha (Inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) assay of endogenous LSD1 protein using LSD1 Antibody: ChIP was performed using LSD1 Polyclonal Antibody (Product # MA5-14934, 5 µg) on sheared chromatin from A549 and A549 cells treated with Dexamethasone (100 nM, 2hours) using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to BIRC3, CDKN1C, DUSP1 (active) and MYOD, SAT alpha (Inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.