MA1-16619

antibody from Invitrogen Antibodies

Targeting: BRCA1 BRCC1, FANCS, PPP1R53, RNF53

Western blot (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16619

Provider

Invitrogen Antibodies

Product name

BRCA1 Monoclonal Antibody (MU)

Antibody type

Monoclonal

Antigen

Other

Description

Suggested positive control: MCF-7 whole cell lysates.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

MU

Vial size

100 μL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BRCA1 in MCF7 cells. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16619) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 650.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BRCA1 in 1 x 10^6 MCF-7 cells. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16619) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BRCA1 in MCF7 cells. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16619) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 650.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of BRCA1 in 1 x 10^6 MCF-7 cells. Samples were incubated in BRCA1 monoclonal antibody (Product # MA1-16619) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.