PA5-18305

antibody from Invitrogen Antibodies

Targeting: FHL1 bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18305

Provider

Invitrogen Antibodies

Product name

FHL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, porcine and rat based on sequence homology.

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of Human Muscle lysate using Product # PA5-18305 at a concentration of 0.05 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FHL1 using FHL1 Polyclonal Antibody (Product # PA5-18305) (0.1 µg/mL) in staining of Mouse Skeletal Muscle (A), Rat Lung (B) and Rat Skeletal Muscle (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin embedded of Human Testis using Product # PA5-18305 at a concentration of 2.5 µg/mL. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FHL1 in human testis. Samples were incubated with FHL1 polyclonal antibody (Product # PA5-18305) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.