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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-28345 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Catenin alpha-1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, A431, mouse brain, rat brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (96%), Rabbit (100%), Rhesus Monkey (99%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Catenin alpha-1 Polyclonal Antibody (Product # PA5-28345). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. Catenin alpha-1 Polyclonal Antibody (Product # PA5-28345) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Catenin alpha-1 was performed by separating 50 µg of Rat tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Catenin alpha-1 Polyclonal Antibody (Product # PA5-28345) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Catenin (Product # PA5-28345) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
