NB100-324

antibody from Novus Biologicals

Targeting: BLM BS, RECQ2, RECQL3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB100-324

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-324, RRID:AB_10001261

Product name

Goat Polyclonal Blooms Syndrome Protein Blm Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate from 293T cells (35 ug for WB; 775 ug for IP). Antibody: Affinity purified goat anti-BLM used at 0.5 ug/ml for WB and 3 ug/mg lysate for IP. Immunoprecipitated BLM was detected by Western Blot using affinity purified rabbit anti-BLM NB100-214. Detection: Chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Detection of human BLM by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-BLM antibody NB100-324 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.