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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [8]
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- Product number
- PA5-27225 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p130Cas Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: H1299, NIH-3T3, 293T, p130Cas shRNA-transfected 293T. Predicted reactivity: Mouse (88%), Rat (88%), Bovine (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.379 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using p130Cas Polyclonal Antibody (Product # PA5-27225). Sample (30 µg of whole cell lysate). Lane A: H1299 . 7.5% SDS PAGE. p130Cas Polyclonal Antibody (Product # PA5-27225) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using p130Cas Polyclonal Antibody (Product # PA5-27225). Sample (30 µg of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. P130Cas Polyclonal Antibody (Product # PA5-27225) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of FABP6 in A549 cells. Sample was incubated with polyclonal FABP6 antibody (Product # PA5-87279) followed by DAPI (nuclear staining).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BCAR1 protein stained by BCAR1 antibody [N2C2], Internal (Product # PA5-27225) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BCAR1 protein stained by BCAR1 antibody [N2C2], Internal (Product # PA5-27225) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of p130Cas was performed in paraffin-embedded mouse brain tissue using p130Cas Polyclonal Antibody (Product # PA5-27225) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of p130Cas was performed in paraffin-embedded rat brain tissue using p130Cas Polyclonal Antibody (Product # PA5-27225) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. BCAR1 antibody [N2C2], Internal (Product # PA5-27225) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of p130Cas was performed in frozen-sectioned adult mouse cerebellum tissue using p130Cas Polyclonal Antibody (Product # PA5-27225) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BCAR1 antibody [N2C2], Internal detects BCAR1 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. BCAR1 antibody [N2C2], Internal (Product # PA5-27225) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of p130Cas was performed in frozen-sectioned adult mouse cerebellum tissue using p130Cas Polyclonal Antibody (Product # PA5-27225) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of p130Cas was performed in paraffin-embedded mouse brain tissue using p130Cas Polyclonal Antibody (Product # PA5-27225) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of p130Cas was performed in paraffin-embedded rat brain tissue using p130Cas Polyclonal Antibody (Product # PA5-27225) at a dilution of 1:500.
