PA1-9040

antibody from Invitrogen Antibodies

Targeting: ERBB3 HER3, LCCS2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-9040

Provider

Invitrogen Antibodies

Product name

ErbB3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ErbB3 in MCF7 cells using a ErbB3 Monoclonal antibody (Product # 45-6600) at 10 µg/mL. Samples were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing plasma membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of ErbB3 using ErbB3 Polyclonal Antibody (Product # PA1-9040) in paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing plasma membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ErbB3 in human lung, respiratory epithelium. Samples were incubated with ErbB3 polyclonal antibody (Product # PA1-9040) using a dilution of 5 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of ErbB3 in MCF7 cells using a ErbB3 monoclonal antibody (Product # PA1-9040) at 10 µg/mL for 1hr, depicted by the blue line. The cells were paraformaldehyde fixed and permeabilized with 0.5% Triton. Primary incubation followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of ErbB3 in MCF7 cells using a polyclonal antibody (Product #PA1-9040). MCF7 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of ErbB3 in MCF7 cells using a polyclonal antibody (Product #PA1-9040). MCF7 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.