PA5-25931

antibody from Invitrogen Antibodies

Targeting: PDK3

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-25931

Provider

Invitrogen Antibodies

Product name

PDK3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 μL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDK3 in MCF-7 cell line lysates. Samples were incubated with PDK3 polyclonal antibody (Product # PA5-25931). Lysates: 35 µg/lane. PDK3 (arrow).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of MCF-7 cells using a PDK3 polyclonal antibody (Product # PA5-25931) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of MCF-7 cells using a PDK3 polyclonal antibody (Product # PA5-25931) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PDK3 in MCF-7 cells (bottom histogram). Samples were incubated with PDK3 polyclonal antibody (Product # PA5-25931) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).