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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-30654 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VPS35 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HCT116, NIH-3T3, JC. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (97%), Chicken (98%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.64 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of VPS35 was performed in HeLa whole cell extracts using 5 µg of VPS35 Polyclonal Antibody (Product # PA5-30654). Samples were transferred to a membrane and probed with VPS35 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using VPS35 (Product # PA5-30654) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of VPS35 was performed in HeLa whole cell extracts using 5 µg of VPS35 Polyclonal Antibody (Product # PA5-30654). Samples were transferred to a membrane and probed with VPS35 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
