MA1-19732

antibody from Invitrogen Antibodies

Targeting: PRKACA PKACa

Western blot

Antibody data

Submit

Validation data

Product number: MA1-19732 - Provider product page
Provider: Invitrogen Antibodies
Product name: PKA alpha Monoclonal Antibody (6D2.1)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: This antibody reacts with human proteinkinase A (an intracellular antigen) catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 6D2.1
Vial size: 100 μg
Concentration: 1 mg/mL
Storage: 4°C, do not freeze

PRKACA protein structure - MA1-19732 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Dot Blot analysis of GST and GST-fusion proteins using a nitrocellulose membrane (5 ng/spot); Lane 1: GST-Akt1, Lane 2: GST-Akt2, Lane 3: GST-Akt3, Lane 4: GST-PKAc alpha, Lane 5: GST-PKAc beta, Lane 6: GST-PKAc gamma, Lane 7: GST-MEK 1, Lane 8: GST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Dot blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1: GST-Akt1; Lane 2: GST-Akt2; Lane 3: GST-Akt3; Lane 4: GST-PKAc alpha Monoclonal antibody (Product # MA1-19732); Lane 5: GST-PKAc beta; Lane 6: GST-PKAc gamma; Lane 7: GST-MEK 1; Lane 8: GST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Dot blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1: GST-Akt1; Lane 2: GST-Akt2; Lane 3: GST-Akt3; Lane 4: GST-PKAc alpha Monoclonal antibody (Product # MA1-19732); Lane 5: GST-PKAc beta; Lane 6: GST-PKAc gamma; Lane 7: GST-MEK 1; Lane 8: GST.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Dot blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05). The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1: GST-Akt1; Lane 2: GST-Akt2; Lane 3: GST-Akt3; Lane 4: GST-PKAc alpha Monoclonal antibody (Product # MA1-19732); Lane 5: GST-PKAc beta; Lane 6: GST-PKAc gamma; Lane 7: GST-MEK 1; Lane 8: GST.