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LearnPA5-18344
antibody from Invitrogen Antibodies
Targeting: FGFR1
BFGFR, CD331, CEK, FLG, FLT2, H2, H3, H4, H5, KAL2, N-SAM
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-18344 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGFR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine and canine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FGFR1 using FGFR1 Polyclonal Antibody (Product # PA5-18344) (0.3 µg/mL) in staining of Human Breast lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-FGFR1 Polyclonal Antibody (Product # PA5-18344) and a 100 kDa band corresponding to FGFR1 was observed across the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of Hep G2 (Lane 1), A549 (Lane 2), HCT 116 (Lane 3), HEK-293 (Lane 4), DU 145 (Lane 5), U-937 (Lane 6) and HT-29 (Lane 7) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG Heavy Chain Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FGFR1 in human kidney. Samples were incubated with FGFR1 polyclonal antibody (Product # PA5-18344) using a dilution of 4 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FGFR1 in human small intestine. Samples were incubated with FGFR1 polyclonal antibody (Product # PA5-18344) using a dilution of 4 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
