PA5-15010

antibody from Invitrogen Antibodies

Targeting: ACHE YT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-15010

Provider

Invitrogen Antibodies

Product name

Acetylcholinesterase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 μL

Concentration

2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Acetylcholinesterase in Jurkat, Raji, Y79 cell line lysates. Samples were incubated with Acetylcholinesterase polyclonal antibody (Product # PA5-15010). Lysates: 35 µg/lane. ACHE protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NCI-H460 cells using an ACHE polyclonal antibody (Product # PA5-15010) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Actin filaments were stained with dye-conjugated phalloidin (red). Nuclei were stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Acetylcholinesterase in NCI-H460 cells. Samples were incubated in Acetylcholinesterase polyclonal antibody (Product # PA5-15010) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Acetylcholinesterase in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with Acetylcholinesterase polyclonal antibody (Product # PA5-15010) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Acetylcholinesterase in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with Acetylcholinesterase polyclonal antibody (Product # PA5-15010) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of NCI-H460 cells using an ACHE polyclonal antibody (Product # PA5-15010) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Acetylcholinesterase in NCI-H460 cells (right histogram). Samples were incubated with Acetylcholinesterase polyclonal antibody (Product # PA5-15010) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).