MA5-15804

antibody from Invitrogen Antibodies

Targeting: AURKA AIK, ARK1, AurA, BTAK, PPP1R47, STK15, STK6, STK7

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15804

Provider

Invitrogen Antibodies

Product name

Aurora A Monoclonal Antibody (4G10)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15804 targets AURKA in indirect ELISA, IHC and WB applications and shows reactivity with Human, Non-human primate, and Rat samples. The MA5-15804 immunogen is purified recombinant fragment of human AURKA expressed in E. Coli. MA5-15804 detects AURKA which has a predicted molecular weight of approximately 48kDa.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

4G10

Vial size

100 μL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AURKA using AURKA monoclonal antibody (Product # MA5-15804) in HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), COS-7 (7) and PC-12 (8) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on nuclear extracts (30 µg lysate) of HCT-116 (Lane 1), HCT-116 treated with Paclitaxel (100 nM, 18 hrs)(Lane 2), HT-29 (Lane 3), HT-29 treated with Paclitaxel (100 nM, 18 hrs) (Lane 4), SK-BR-3 (Lane 5), SK-BR-3 treated with Nocodazole (100 ng/mL, 16 hrs) (Lane 6). The blot was probed with Aurora A Monoclonal Antibody (4G10)(Product #MA51-5804, 1:2,000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4,000 dilution). A 48 kDa band corresponding to Aurora A was observed upon the indicated treatments in the cell lines. The bands marked with * at 40 kDa and 35k Da may represent Aurora B and C respectively, however, their identity could not be confirmed.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and lung cancer (right) using AURKA monoclonal antibody (Product # MA5-15804) followed with DAB staining.