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LearnPA5-17233
antibody from Invitrogen Antibodies
Targeting: APEX1
APE, APE-1, APEN, APEX, APX, HAP1, REF-1, REF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-17233 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-APE1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ape1 in extracts from H1975 and HCC827 cells using Ape1 polyclonal antibody (Product # PA5-17233).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of APE1 was achieved by transfecting HeLa cells with APE1 specific siRNAs (Silencer® select Product # s1445). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) APE1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with APE1 Polyclonal Antibody (Product # PA5-17233, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1 mg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to APE1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), LNCaP (Lane 3), PC-3 (Lane 4), A549 (Lane 5), A-431 (Lane 6), COLO 205 (Lane 7), HT-29 (Lane 8), Panc-1 (Lane 9) and NIH/3T3 (Lane 10). The blot was probed with Anti-APE1 Polyclonal Antibody (Product # PA5-17233, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 38 kDa band corresponding to APE1 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ape1 in normal mouse hippocampus using an Ape1 polyclonal antibody (Product # PA5-17233) (green) and a Neurofilament-H monoclonal antibody (red). DNA is labeled using a fluorescent blue dye.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ape1 in normal mouse cerebellum using an Ape1 polyclonal antibody (Product # PA5-17233) (green) and a Neurofilament-H monoclonal antibody (red). DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous APE1 protein at specific gene loci using Anti-APE1 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-APE1 Rabbit Polyclonal Antibody (Product # PA5-17233, 6 µl) on sheared chromatin from 2 million HeLa cells treated with Methyl methanesulphonate (1mM for 2 hours) using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over MDR1, GAPDH promoter and gene, ACTB promoter and gene (active) and SAT2 satellite repeats (inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
