PA5-19958

antibody from Invitrogen Antibodies

Targeting: BNIP3L BNIP3a, Nix

Western blot

Antibody data

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Validation data

Product number: PA5-19958 - Provider product page
Provider: Invitrogen Antibodies
Product name: BNIP3L Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: In Western blot, an upper band is detected at ~40kDa, which represents the monomer of Bnip3L. A suggested positive control is K562 cell lysate. PA5-19958 can be used with blocking peptide PEP-0083.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 μg
Concentration: 1 mg/mL
Storage: 4°C

BNIP3L protein structure - PA5-19958 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of BNIP3L was performed using 70% confluent log phase HeLa cells treated with Cobalt chloride. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PHD1 Recombinant Rabbit Monoclonal Antibody (3G4) (Product # PA5-19958) at 5 µg/mL dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing increase in Nuclear localization. Panel e represents untreated cells with Nuclear localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of BNIP3L was performed using 70% confluent log phase HeLa cells treated with Cobalt chloride. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PHD1 Recombinant Rabbit Monoclonal Antibody (3G4) (Product # PA5-19958) at 5 µg/mL dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing increase in Nuclear localization. Panel e represents untreated cells with Nuclear localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.