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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-13830 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Creatine Kinase MB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, porcine and rabbit based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 1.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Creatine Kinase MB in C6 cell lysate. Samples were incubated with Creatine Kinase MB polyclonal antibody (Product # PA5-13830).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Creatine Kinase MB in formalin-fixed and paraffin-embedded human skeletal muscle. Samples were incubated with Creatine Kinase MB polyclonal antibody (Product # PA5-13830) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Creatine Kinase MB in formalin-fixed and paraffin-embedded human skeletal muscle. Samples were incubated with Creatine Kinase MB polyclonal antibody (Product # PA5-13830) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using a Creatine Kinase MB polyclonal antibody (Product # PA5-13830) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Creatine Kinase MB in HepG2 cells (bottom histogram). Samples were incubated with Creatine Kinase MB polyclonal antibody (Product # PA5-13830) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).
