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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MA1-41115 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKT2 Monoclonal Antibody (95C657.1.2)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: HL-60 whole cell lysate, human placenta or HL-60, human Placenta protein, antigen standard for AKT2 (transient overexpression lysate). Immunogen sequence: VANSLKQRAP GEDPMDYK
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- 95C657.1.2
- Vial size
- 200 μL
- Concentration
- Conc. Not Determined
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Flow cytometry of AKT2 in A549 cells. Samples were incubated in AKT2 monoclonal antibody (Product # MA1-41115) using a dilution of 1.0 µg/mL for 30 minutes at room temperature followed by Mouse IgM (H+L) Cross-Adsorbed Secondary Antibody, Dylight 488. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.
