- PA5-12511 - Invitrogen Antibodies ENG antibody

Submitted references Hepatocyte-Specific Deletion of HIF2α Prevents NASH-Related Liver Carcinogenesis by Decreasing Cancer Cell Proliferation.

Foglia B, Sutti S, Cannito S, Rosso C, Maggiora M, Autelli R, Novo E, Bocca C, Villano G, Ramavath NN, Younes R, Tusa I, Rovida E, Pontisso P, Bugianesi E, Albano E, Parola M
Cellular and molecular gastroenterology and hepatology 2022;13(2):459-482

Quantitative Analysis of SSEA3+ Cells from Human Umbilical Cord after Magnetic Sorting.

Leng Z, Sun D, Huang Z, Tadmori I, Chiang N, Kethidi N, Sabra A, Kushida Y, Fu YS, Dezawa M, He X, Young W
Cell transplantation 2019 Jul;28(7):907-923

Trichinella Spiralis Impact on Mesenchymal Stem Cells: Immunohistochemical Study by Image Analyzer in Murine Model.

Hasby Saad MA, Hasby EA
Experimental and molecular pathology 2017 Jun;102(3):396-407

Endoglin (CD105) coordinates the process of endometrial receptivity for embryo implantation.

Chadchan SB, Kumar V, Maurya VK, Soni UK, Jha RK
Molecular and cellular endocrinology 2016 Apr 15;425:69-83

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of CD105 in formalin fixed and paraffin embedded mouse heart tissue. Samples were incubated with CD105 polyclonal antibody (Product # PA5-12511) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of NCI-H292 cells using a CD105 polyclonal antibody (Product # PA5-12511) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of CD105 in NCI-H292 cells (bottom histogram). Samples were incubated with CD105 polyclonal antibody (Product # PA5-12511) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (top histogram).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Hepatocyte-specific deletion of HIF-2alpha does not affect HIF-1alpha expression. Liver expression of HIF-1alpha evaluated by ( A ) Q-PCR and ( B ) immunohistochemical analysis in HCCs from 9 WT or from 6 hHIF-2alpha -/- . Original magnification is indicated. ( C ) Gene expression of vascular endothelial growth factor (VEGF), FLK1, and vascular endothelialcadherin evaluated by Q-PCR in HCC tumor masses from 9 WT mice or from 6 hHIF-2alpha -/- mice ( C ). The mRNA values are expressed as the fold increase over control values after normalization to the TATA box binding protein gene expression. Results are expressed as means +- SD. Boxes include the values within the 25th and 75th percentiles, whereas the horizontal bars represent the medians. The extremities of the vertical bars (10th-90th percentile) comprise 80% of the values. Statistical differences were assessed by the Student t test or the Mann-Whitney test for nonparametric values. ( D ) WB analysis of cd105 protein levels in HCCs from 7 WT mice or from 5 hHIF-2alpha -/- mice. For the Western blot analysis, Bio-Rad Quantity One software was used to perform the densitometric analysis. Equal loading was evaluated by reprobing membranes for beta-actin. Statistical differences were assessed by the Student t test or the Mann-Whitney test for nonparametric values.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 12. Muse cell clusters on polyHEMA-coated dishes. (A) On day 2 after seeding the cells, clusters had formed but were no more than 50 mum in diameter. (B) On day 7, many clusters had formed with a diameter of approximately 100 mum. (C) Immunocytochemistry of the clusters that were transferred onto coverslips and cultured for 8 h. (C1) CD105 (red), (C2) SSEA3 (green), (C3) Hoechst 33342 (blue), (C4) Merge. Almost all the cells were CD105+, and approximately half were SSEA3+. (D, E) Two images obtained under a Zeiss confocal 510 microscope at 40X showing the sorted Muse cells after culture for 2 days. Scale bars: 50 um (A, C), 100 um (B), 10 um (D), and 20 um (E).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 5. Immunofluorescence images of CD105 (red), SSEA3 (green), and Hoechst 33342 (blue) staining of cultured cells from 96WJP2. (A1) CD105-labeled cells; (A2 )some giant SSEA3+ cells with long and very thin processes contacting each other; (A3) nuclei stained with Hoechst 33342.; (A4) merged images. (B) CD105+ and SSEA3+ cells that appear to have just completed dividing. A Zeiss confocal 510 microscope was used to take these pictures at 40X. Scale bar, 20 mum (A) or 5 mum (B).

PA5-12511

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