PA5-20012

antibody from Invitrogen Antibodies

Targeting: AICDA AID, ARP2, CDA2, HIGM2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20012

Provider

Invitrogen Antibodies

Product name

AID Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is Ramos cell lysate. PA5-20012 can be used with blocking peptide PEP-0131.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ-on-a-Chip.

Goyal G, Prabhala P, Mahajan G, Bausk B, Gilboa T, Xie L, Zhai Y, Lazarovits R, Mansour A, Kim MS, Patil A, Curran D, Long JM, Sharma S, Junaid A, Cohen L, Ferrante TC, Levy O, Prantil-Baun R, Walt DR, Ingber DE
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 May;9(14):e2103241

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of AID in Ramos cells with AID Polyclonal Antibody (Product # PA5-20012) at 10 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of AID in Ramos cells with AID Polyclonal Antibody (Product # PA5-20012) at 20 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of AID in Ramos cells with AID Polyclonal Antibody (Product # PA5-20012) at 10 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of AID in Ramos cells with AID Polyclonal Antibody (Product # PA5-20012) at 20 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 B cells remain quiescent yet express AID in LFs formed on-chip. a) Representative flow cytometric characterization of B cells stained for IgD and CD27 in the initial PBMC sample (PBMC; n = 3) compared with cells from explanted tonsils (Tonsil; n = 2), or cells cultured in the LF chip for 4 days (LF Chip; n = 3). The percentage of cells in the positive peak is indicated above the gate drawn on the histogram. b) Representative confocal immunofluorescence micrograph showing AID expression (green) in B cells cultured in the LF chip for 4 days (similar results are obtained with 4 donors); Hoechst stained nuclei in the lymphocytes are shown in blue. c) Quantification of AID expression levels in 183 particles ranging from single cell sized regions-of-interest to large follicles from 4 independent fields of view measured as MFI plotted as a function of particle size (Particle area) in perfused Organ Chips cultured for 4 days. Each data point represents 1 particle and particles from 4 images from the LF chips created from one representative donor shown. Similar results obtained in 3 additional donors. Non-parametric Spearman correlation shown.