PA5-29669

antibody from Invitrogen Antibodies

Targeting: ITK EMT, LYK, PSCTK2

Western blot

Antibody data

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Validation data

Product number: PA5-29669 - Provider product page
Provider: Invitrogen Antibodies
Product name: ITK Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: Molt-4, Jurkat. Predicted reactivity: Mouse (92%), Rat (93%), Rhesus Monkey (98%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 0.39 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ITK protein structure - PA5-29669 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of ITK was performed in Jurkat cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK Polyclonal Antibody (Product # PA5-29669) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Tyrosine-protein kinase ITK/TSK was performed using Jurkat and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ITK Polyclonal Antibody (Product # PA5-29669) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membrane staining for ITK in Jurkat cells but not in Raji (Panel e). Panel f represents control Jurkat cells with no primary antibody to assess background. The images were captured at 60X magnification. ITK expression is reported to be seen in T cells (Jurkat) and natural killer cells but not in B cells (Raji).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of ITK was performed in Jurkat cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ITK Polyclonal Antibody (Product # PA5-29669) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Tyrosine-protein kinase ITK/TSK was performed using Jurkat and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ITK Polyclonal Antibody (Product # PA5-29669) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membrane staining for ITK in Jurkat cells but not in Raji (Panel e). Panel f represents control Jurkat cells with no primary antibody to assess background. The images were captured at 60X magnification. ITK expression is reported to be seen in T cells (Jurkat) and natural killer cells but not in B cells (Raji).