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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PA1-4613 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ATF2 (Ser490, Ser498) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat based on 100% sequence homology. This antibody is specific for ~74 kDa ATF2 protein phosphorylated at Ser490,498. It also recognizes the phosphorylated ~54 kDa splice form of ATF2. Store at -20ºC short term, 80ºC long term.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references RhoJ regulates melanoma chemoresistance by suppressing pathways that sense DNA damage.
Ho H, Aruri J, Kapadia R, Mehr H, White MA, Ganesan AK
Cancer research 2012 Nov 1;72(21):5516-28
Cancer research 2012 Nov 1;72(21):5516-28
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of ATF2 in human melanoma cells incubated with varying doses of the radiomimetic drug NCS, showing specific immunolabeling of a band at ~74 kDa corresponding to Phospho-ATF2 (Ser490, Ser498) polyclonal antibody (Product # PA1-4613).
