MA5-15435

antibody from Invitrogen Antibodies

Targeting: EPHA4 Hek8, TYRO1

ELISA

Antibody data

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Validation data

Product number: MA5-15435 - Provider product page
Provider: Invitrogen Antibodies
Product name: EphA4 Monoclonal Antibody (6C1B6)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15435 targets EphA3 in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15435 immunogen is purified recombinant fragment of EphA3 (aa751-983) expressed in E. Coli..
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 6C1B6
Vial size: 100 μL
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

EPHA4 protein structure - MA5-15435 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of EphA4 was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA overnight at room temperature. The cells were labeled with EphA4 Monoclonal Antibody (6C1B6) (Product # MA5-15435) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic (endosome-like) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of EphA4 was performed using Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA overnight at room temperature. The cells were labeled with EphA4 Monoclonal Antibody (6C1B6) (Product # MA5-15435) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic (endosome-like) localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.