PA5-32754

antibody from Invitrogen Antibodies

Targeting: EPHA4 Hek8, TYRO1

Antibody data

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Validation data

Product number: PA5-32754 - Provider product page
Provider: Invitrogen Antibodies
Product name: EphA4 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%); Dog, Panda, Horse (94%); Bovine, Bat, Hamster, Elephant, Pig, Opossum, Turkey, Chicken (88%); Mouse, Rat, Rabbit, Platypus, Lizard, Xenopus (81%). For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 50 μg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

EPHA4 protein structure - PA5-32754 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of EPHA4 was performed using NSC and NSCs differentiated to neurons. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EphA4 Polyclonal Antibody (Product # PA5-32754, 1:200) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing axonal and dendritic localization. Panel e represents NSC showing very low expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of EPHA4 was performed using NSC and NSCs differentiated to neurons. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EphA4 Polyclonal Antibody (Product # PA5-32754, 1:200) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withRhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing axonal and dendritic localization. Panel e represents NSC showing very low expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.