PA5-21166

antibody from Invitrogen Antibodies

Targeting: APOA1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21166

Provider

Invitrogen Antibodies

Product name

ApoA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is chicken liver lysate. PA5-21166 can be used with blocking peptide PEP-1280.

Reactivity

Human, Chicken/Avian

Host

Rabbit

Isotype

IgG

Vial size

100 μg

Concentration

1 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

References

Submitted references

Caspase-3 Cleaves Extracellular Vesicle Proteins During Auditory Brainstem Development.

Weghorst F, Mirzakhanyan Y, Samimi K, Dhillon M, Barzik M, Cunningham LL, Gershon PD, Cramer KS
Frontiers in cellular neuroscience 2020;14:573345

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Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer.

Justesen BH, Hansen RW, Martens HJ, Theorin L, Palmgren MG, Martinez KL, Pomorski TG, Fuglsang AT
The Journal of biological chemistry 2013 Sep 13;288(37):26419-29

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ApoA1 in chicken liver tissue lysate with ApoA1 Polyclonal Antibody (Product # PA5-21166) at 1 µg/mL .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 The purity of EV preparation. (A) Western blot comparing protein levels in three biological replicates of ABEVs (EV) and crude brainstem lysates (BS). Neural cell adhesion molecule (NCAM), a positive marker for EVs released by neural tissue, was enriched in ABEVs. In contrast, negative markers for EVs, namely calnexin (CANX), APOA1, and VDAC, showed either no difference in protein amount between brainstem lysates and ABEVs (CANX and APOA1) or showed a decreased expression in ABEVs (VDAC). Both procaspase-3 (PC3) and cleaved caspase-3 (CC3) were negatively enriched in ABEVs compared to brainstem lysates. (B) All proteins were normalized to total protein stain as a loading control. (C) Quantification of normalized protein levels from A. All p -values are derived from two-tailed Student's t -tests except for procaspase-3, for which Welch's heteroscedastic t -test was necessary. (D) Proportionally accurate Venn diagram of proteins identified in each ABEV replicate. The substantial triple-overlap reflects a high degree of conservation among the proteomes of the individual ABEV replicates. (E) Comparison of ExoCarta dataset counts of brainstem proteins and ABEV proteins found in one, two, or three replicates. The number of EV proteomic datasets containing each protein on the EV database ExoCarta (""ExoCarta dataset counts"") was used to estimate the frequency that proteins are found in EVs. ABEV proteins had higher ExoCarta dataset counts than brainstem proteins regardless