PA5-27824

antibody from Invitrogen Antibodies

Targeting: ACO1 IREB1, IREBP, IRP1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27824

Provider

Invitrogen Antibodies

Product name

Aconitase 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (93%), Rat (93%), Zebrafish (81%), Xenopus laevis (85%), Rabbit (94%), Chicken (85%), Rhesus Monkey (99%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.78 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Tumor-initiating cells of breast and prostate origin show alterations in the expression of genes related to iron metabolism.

Rychtarcikova Z, Lettlova S, Tomkova V, Korenkova V, Langerova L, Simonova E, Zjablovskaja P, Alberich-Jorda M, Neuzil J, Truksa J
Oncotarget 2017 Jan 24;8(4):6376-6398

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Aconitase 1 was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: Aconitase 1 Polyclonal Antibody (Product # PA5 27824) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Aconitase 1 was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: Aconitase 1 Polyclonal Antibody (Product # PA5 27824) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.