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LearnPA5-20398
antibody from Invitrogen Antibodies
Targeting: IL1RL1
DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-20398 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-33 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The PA5-20398 immunogen is located within the 150-200 amino acids of IL-33. Predicted molecular ~ 31kD. In Western blot applications, this antibody has been observed to detect a band at: 33kD. Despite its predicted molecular weight, IL-33 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is human lymph node tissue lysate. PA5-20398 can be used with blocking peptide PEP-0515. Predicted species reactivity based on immunogen sequence: Rat: (76.5%)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human lymph node cells using a IL-33 polyclonal antibody (Product # PA5-20398) at a 20 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lymph node tissue using IL-33 Polyclonal Antibody (Product # PA5-20398) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed human lymph node tissue labeling IL-33 with IL-33 Polyclonal Antibody (Product # PA5-20398) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
