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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-11325 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ubiquitin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, canine, equine, hamster, mouse, porcine, non-human primate, rat, rabbit and Xenopus and yeast based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ubiquitin in HeLa cell lysate. Samples were incubated with Ubiquitin polyclonal antibody (Product # PA5-11325) followed by HRP-anti-rabbit (used for signal visualization with chemiluminescence). Ubiquitin (Arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ubiquitin co-localized with FOXP2.10t using an antibody against FOXP2 (green) counterstained with a Ubiquitin polyclonal antibody (Product # PA5-11325) (red), indicating that the cellular bodies represent aggresomes. Nuclei are marked by DAPI staining (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Ubiquitin in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with Ubiquitin polyclonal antibody (Product # PA5-11325) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Ubiquitin in formalin-fixed and paraffin-embedded human cancer tissue. Samples were incubated with Ubiquitin polyclonal antibody (Product # PA5-11325) which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
