PA1-4686

antibody from Invitrogen Antibodies

Targeting: SNCA NACP, PARK1, PARK4, PD1

Western blot (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-4686

Provider

Invitrogen Antibodies

Product name

Phospho-alpha Synuclein (Ser129) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, human, mouse and non-human primate based on 100% sequence homology. This antibody is specific for the ~15 kDa alpha-synuclein protein phosophorylated at Ser129. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.25 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Trehalose does not improve neuronal survival on exposure to alpha-synuclein pre-formed fibrils.

Redmann M, Wani WY, Volpicelli-Daley L, Darley-Usmar V, Zhang J
Redox biology 2017 Apr;11:429-437

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Wild type and P301L mutant Tau promote neuro-inflammation and α-Synuclein accumulation in lentiviral gene delivery models.

Khandelwal PJ, Dumanis SB, Herman AM, Rebeck GW, Moussa CE
Molecular and cellular neurosciences 2012 Jan;49(1):44-53

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 PFFs induce high molecular weight (HMW) and insoluble aggregates. (A) Western blot analysis of total monomeric and HMW alphaSyn from primary cortical neurons treated for 14 days with PFFs. (B, C) Quantification of western blots from A. (D) Western blot analysis of total and monomeric alphaSyn of primary cortical neurons exposed to alphaSyn monomer for 14 days. (E, F) Quantification of western blots D. Data represent mean+-SEM (n=3). * p