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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-29315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HeLa, HepG2 , HCT116. Predicted reactivity: Mouse (95%), Rat (96%), Chicken (82%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EphA1 Polyclonal Antibody (Product # PA5-29315). Sample (30 µg of whole cell lysate). Lane A: HepG2. Lane B: HCT116 . 7.5% SDS PAGE. EphA1 Polyclonal Antibody (Product # PA5-29315) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EphA1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EphA1 Polyclonal Antibody (Product # PA5-29315) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
