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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- MA5-15239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Chk1 Monoclonal Antibody (2G1D5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15239 targets CHK1 in indirect ELISA, WB applications and shows reactivity with Human and mouse samples. The MA5-15239 immunogen is purified recombinant fragment of CHK1 expressed in E. Coli. MA5-15239 detects CHK1 which has a predicted molecular weight of approximately 56kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G1D5
- Vial size
- 100 μg
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHK1 using CHK1 monoclonal antibody (Product # MA5-15239) in A431 (1), HeLa (2), NIH/3T3 (3) and K562 (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHK1 using CHK1 monoclonal antibody (Product # MA5-15239) in HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CHK1 cDNA (2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Chk1 was achieved by transfecting HeLa with Chk1 specific siRNAs (Silencer® select Product # s504). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Chk1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Chk1 Monoclonal Antibody (2G1D5) (Product # MA5-15239, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Chk1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Chk1 Monoclonal Antibody (2G1D5) (Product # MA5-15239) and a 55kDa band corresponding to Chk1 was observed across cell lines tested. Whole cell extracts (30µg lysate) of HeLa (Lane 1), SH-SY5Y (Lane 2), HCT 116 (Lane 3), A549 (Lane 4) and K-562 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
