PA5-19131

antibody from Invitrogen Antibodies

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19131

Provider

Invitrogen Antibodies

Product name

IL-18 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Molecular Characteristics, Clinical Implication, and Cancer Immunity Interactions of Pyroptosis-Related Genes in Breast Cancer.

Xu D, Ji Z, Qiang L
Frontiers in medicine 2021;8:702638

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CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation.

Mishra SK, Gao YG, Deng Y, Chalfant CE, Hinchcliffe EH, Brown RE
Autophagy 2018;14(5):862-879

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of IL-18 using IL-18 Polyclonal Antibody (Product # PA5-19131) in paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of IL-18 using IL-18 Polyclonal Antibody (Product # PA5-19131) in paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing Golgi and cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of IL-18 using IL-18 Polyclonal Antibody (Product # PA5-19131) in paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9 Validation of the expression of GSDMC, IL-18 , and TIRAP in normal breast cells (MCF-10A) and breast cancer cells (MDA-MB-231 and HCC70). (A-D) Western blot detecting the expression of GSDMC, IL-18, and TIRAP in MCF-10A, MDA-MB-231, and HCC70 cells. *** p < 0.001; **** p < 0.0001 . (E-G) Immunofluorescence of the expression of GSDMC, IL-18, and TIRAP in MCF-10A, MDA-MB-231, and HCC70 cells. Scale bar, 5 mum; magnification 200 x.