PA5-19872

antibody from Invitrogen Antibodies

Targeting: ADAM17 CD156B, cSVP, TACE

Western blot

Antibody data

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Validation data

Product number: PA5-19872 - Provider product page
Provider: Invitrogen Antibodies
Product name: ADAM17 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control is Hela cell lysate. A 17 amino acid peptide near the carboxy terminus of human TACE. The PA5-19872 immunogen is located within the last 50 amino acids of TACE. Predicted molecular ~ 93kD. In Western blot applications, this antibody has been observed to detect a band at: 93-125kD (Post-modification: 9 N-linked glycosylation). Predicted species reactivity based on immunogen sequence: mouse (94.1%).
Reactivity: Human, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μg
Concentration: 1 mg/mL
Storage: Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

ADAM17 protein structure - PA5-19872 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of HeLa cells using a CD156b/ADAM17/TACE polyclonal antibody (Product # PA5-19872) at a 10 µg/mL dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of HeLa cells using ADAM17 Polyclonal Antibody (Product # PA5-19872) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with ADAM17 Polyclonal Antibody (Product # PA5-19872) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with ADAM17 Polyclonal Antibody (Product # PA5-19872) at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of HeLa cells using ADAM17 Polyclonal Antibody (Product # PA5-19872) at 10 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.