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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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- Product number
- PA5-12942 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-B-Raf (Thr400) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot analysis of Phospho-BRAF pThr400 polyclonal antibody (Product # PA5-12942) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg per mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot analysis of Phospho-B-Raf (Thr400). Samples were incubated with Phospho-B-Raf (Thr400) polyclonal antibody (Product # PA5-12942) using a dilution of 0.5 µg/mL. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed on nitrocellulose membrane.
