MA1-19174

antibody from Invitrogen Antibodies

Targeting: ALB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

MA1-19174

Provider

Invitrogen Antibodies

Product name

Human Serum Albumin Monoclonal Antibody (AL-01)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts only with human serum albumin, a 65-67 kDa monomeric protein in human blood plasma; it is produced in liver. No cross-reactivity was observed with other serum proteins.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

AL-01

Vial size

100 μg

Concentration

1 mg/mL

Storage

4°C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis (reducing conditions) of human serum albumin using anti-human Albumin (AL-01) Monoclonal antibody (Product # MA1-19174). Lane 1: Human blood plasma; Lane 2: Human blood serum.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Human Serum Albumin Monoclonal Antibody (AL-01) (Product # MA1-19174) and a 66kDa band corresponding to Human Serum Albumin was observed in Hep G2 when compared to HCT 116 which is reported to be a low expressing model. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with PTI (1X for 4hrs) (Lane 2), HCT 116 (Lane 3), HCT 116 PTI (1X for 4hrs) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).