MA5-15451

antibody from Invitrogen Antibodies

Targeting: ALB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA5-15451

Provider

Invitrogen Antibodies

Product name

Human Serum Albumin Monoclonal Antibody (2H3D1;8F6F9)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-15451 targets human Albumin in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15451 immunogen is human sera albumin. MA5-15451 detects human Albumin which has a predicted molecular weight of approximately 69kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2H3D1;8F6F9

Vial size

100 μL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Albumin using human Albumin monoclonal antibody (Product # MA5-15451) in human serum (lane 1, 3) and plasma (lane 2, 4).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Human Serum Albumin Monoclonal Antibody (2H3D1;8F6F9)(Product # MA5-15451) and a 66kDa band corresponding to Human Serum Albumin was observed across in Hep G2 when compared to HCT 116 which is reported to be a low expressing model. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with PTI (1X for 4hrs) (Lane 2), HCT 116 (Lane 3), HCT 116 treated with PTI (1X for 4hrs) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).