MA5-14366

antibody from Invitrogen Antibodies

Targeting: CDK5 PSSALRE

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14366

Provider

Invitrogen Antibodies

Product name

Cdk5 Monoclonal Antibody (DC34)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-14366 targets Cdk5 in immunofluorescence, immunoprecipitation, and Western blot applications and shows reactivity with human, mouse, and rat samples. The MA5-14366 immunogen is recombinant cdk5 protein.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

DC34

Vial size

500 μL

Concentration

0.2 mg/mL

Storage

4°C

References

Submitted references

(+/-)-huprine Y, (-)-huperzine A and tacrine do not show neuroprotective properties in an apoptotic model of neuronal cytoskeletal alteration.

Jordá EG, Verdaguer E, Jiménez A, Canudas AM, Rimbau V, Camps P, Muñoz-Torrero D, Camins A, Pallàs M
Journal of Alzheimer's disease : JAD 2004 Dec;6(6):577-83; discussion 673-81

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cdk5 (green) showing staining in the cytoplasm of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk5 monoclonal antibody (Product # MA5-14366) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Cdk5 (green) showing staining in the cytoplasm of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cdk5 monoclonal antibody (Product # MA5-14366) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Cdk5 using Cdk5 Monoclonal Antibody (MA5-14366) on Native Human LS174T Cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Cdk5 using Cdk5 Monoclonal Antibody (MA5-14366) on Native Human LS174T Cells.