PA5-27693

antibody from Invitrogen Antibodies

Targeting: MAP2K1 MAPKK1, MEK1, PRKMK1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27693

Provider

Invitrogen Antibodies

Product name

MEK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (95%), Dog (100%), Pig (95%), Rabbit (100%), Chicken (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MEK1/MAP2K1 in paraformaldehyde-fixed HeLa cells using a MEK1/MAP2K1 polyclonal antibody (Product # PA5-27693) (Green) at a 1:200 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of MEK1 was performed in paraffin-embedded rat liver tissue using MEK1 Polyclonal Antibody (Product # PA5-27693) at a dilution of 1:500.