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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-15629 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK1 Monoclonal Antibody (8C5A6)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15629 targets CDC2 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15629 immunogen is purified recombinant fragment of CDC2 expressed in E. Coli. MA5-15629 detects CDC2 which has a predicted molecular weight of approximately 34kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8C5A6
- Vial size
- 100 μL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Rigosertib and Cholangiocarcinoma: A Cell Cycle Affair.
Malacrida A, Cavaletti G, Miloso M
International journal of molecular sciences 2021 Dec 25;23(1)
International journal of molecular sciences 2021 Dec 25;23(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and esophageal cancer (B) using CDC2 monoclonal antibody (Product # MA5-15629) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Western blot analysis of P-CDK1 and PLK1 in EGI-1 cells treated with Rig. ( A ) Representative images of P-CDK1, PLK1 and actin after treatment with different concentrations of 100 nM Rig for 24 and 48 h. ( B ) The graph represents the quantification of P-CDK1 Western blot, normalized to actin. ( C ) The graph represents the quantification of PLK1 western blot, normalized to actin. All the graphs are represented as the mean percentage +- SD of at least three independent experiments and are compared to untreated controls arbitrarily set to 100%. * p < 0.05 vs. CTRL.
