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antibody from Invitrogen Antibodies
Targeting: RELA
NFKB3, p65
Western blot
Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-27617 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFkB p65 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, HeLa, HepG2, K562, THP-1, HL-60, human NFkB p65-transfected 293T. Predicted reactivity: Mouse (96%), Rat (99%), Pig (98%), Chimpanzee (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.49 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Diacerein counteracts acetaminophen-induced hepatotoxicity in mice via targeting NLRP3/caspase-1/IL-1β and IL-4/MCP-1 signaling pathways.
Elshal M, Abdelmageed ME
Archives of pharmacal research 2022 Mar;45(3):142-158
Archives of pharmacal research 2022 Mar;45(3):142-158
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NFkB p65 in paraformaldehyde-fixed U2OS cells using a NFkB p65 polyclonal antibody (Product # PA5-27617) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFkB p65 Polyclonal Antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. NFkB p65 stained by NFkB p65 Polyclonal Antibody (Product # PA5-27617) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFkB p65 Polyclonal Antibody detects NFkB p65 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NFkB p65 stained by NFkB p65 Polyclonal Antibody (Product # PA5-27617) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFkB p65 Polyclonal Antibody detects NFkB p65 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. NFkB p65 Polyclonal Antibody (Product # PA5-27617) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
