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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-12607 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-ErbB4 (Tyr1188) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-ErbB4 (Tyr1188) in lysates from A431 cell line, untreated or treated with EGF, 100 ng/mL. Samples were incubated with Phospho-ErbB4 (Tyr1188) polyclonal antibody (Product # PA5-12607). Phospho-HER4 (Y1188) Antibody (upper) or tubulin (lower).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-ErbB4 (Tyr1188) in paraffin-embedded Human breast carcinoma tissue. Samples were incubated with Phospho-ErbB4 (Tyr1188) polyclonal antibody (Product # PA5-12607) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-ErbB4 (Tyr1188) in paraffin-embedded Human kidney tissue. Samples were incubated with Phospho-ErbB4 (Tyr1188) polyclonal antibody (Product # PA5-12607) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-ErbB4 (Tyr1188) in paraffin-embedded Human breast carcinoma tissue. Samples were incubated with Phospho-ErbB4 (Tyr1188) polyclonal antibody (Product # PA5-12607) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-ErbB4 (Tyr1188) in paraffin-embedded Human kidney tissue. Samples were incubated with Phospho-ErbB4 (Tyr1188) polyclonal antibody (Product # PA5-12607) using a dilution of 1:100 for 1 hour at room temperature followed by undiluted CRF Anti-Polyvalent HRP Polymer antibody. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH 9 0).
