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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29272 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ErbB4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A431, mouse brain, rat brain. Predicted reactivity: Mouse (89%), Rat (97%), Xenopus laevis (86%), Chicken (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (Product # PA5-29272) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (Product # PA5-29272) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (Product # PA5-29272) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Her4 / ErbB4 protein from MCF-7 whole cell extract using 4.5 µg of ErbB4 Polyclonal Antibody (Product # PA5-29272). Western blot analysis was performed using ErbB4 Polyclonal Antibody (Product # PA5-29272) and diluted at 1:500. HRP-conjugated anti rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ERBB4 (v-erb-A) (Product # PA5-29272) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Her4 / ErbB4 protein from MCF-7 whole cell extract using 4.5 µg of ErbB4 Polyclonal Antibody (Product # PA5-29272). Western blot analysis was performed using ErbB4 Polyclonal Antibody (Product # PA5-29272) and diluted at 1:500. HRP-conjugated anti rabbit IgG antibody was used to detect the primary antibody.
