PA5-29932

antibody from Invitrogen Antibodies

Targeting: ATP6AP2 APT6M8-9, ATP6IP2, ATP6M8-9, M8-9, PRR, RENR

Antibody data

Submit

Validation data

Product number: PA5-29932 - Provider product page
Provider: Invitrogen Antibodies
Product name: ATP6IP2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (92%), Rat (91%), Rhesus Monkey (98%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 1.38 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ATP6AP2 protein structure - PA5-29932 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ATP6IP2 Polyclonal Antibody (Product # PA5-29932). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP6IP2 Polyclonal Antibody (Product # PA5-29932) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-ATP6IP2 Polyclonal Antibody (Product # PA5-29932) and a ~37 kD band was observed across the panel tested. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), PC-3 (Lane 3), SiHa (Lane 4), Panc-1 (Lane 5) and HCT 116 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of ATP6IP2 was achieved by transfecting MCF-7 with ATP6IP2 specific siRNAs (Silencer® select Product # s19791, 19790). Western blot analysis (Fig. a) was performed using whole cell extracts from the ATP6IP2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Arp3 Polyclonal Antibody (Product # PA5-29932, 1:1,000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP6IP2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Renin Receptor in methanol-fixed HepG2 cells using a Renin Receptor polyclonal antibody (Product # PA5-29932) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Renin Receptor antibody detects Renin Receptor protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Renin Receptor stained by Renin Receptor antibody (Product # PA5-29932) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ATP6IP2 Polyclonal Antibody detects Renin Receptor protein by immunofluorescent analysis. Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Renin Receptor stained by ATP6IP2 Polyclonal Antibody (Product # PA5-29932) diluted at 1:500. Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Renin Receptor antibody detects Renin Receptor protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Renin Receptor stained by Renin Receptor antibody (Product # PA5-29932) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ATP6IP2 Polyclonal Antibody detects Renin Receptor protein by immunofluorescent analysis. Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Renin Receptor stained by ATP6IP2 Polyclonal Antibody (Product # PA5-29932) diluted at 1:500. Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .