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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-13700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Biglycan Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Biglycan in 293 cell lysates. Samples were incubated with Biglycan polyclonal antibody (Product # PA5-13700). Lysates: 2 µg/lane. 293 cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BGN gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Biglycan in SK-Br-3 cell line lysates. Samples were incubated with Biglycan polyclonal antibody (Product # PA5-13700). Lysates: 35 µg/lane. BGN (arrow).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Biglycan in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with Biglycan polyclonal antibody (Product # PA5-13700) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of A375 cells using a BGN polyclonal antibody (Product # PA5-13700) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Biglycan in A375 cells (right histogram). Samples were incubated with Biglycan polyclonal antibody (Product # PA5-13700) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
