PA5-18606

antibody from Invitrogen Antibodies

Targeting: BGN DSPG1, SLRR1A

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-18606

Provider

Invitrogen Antibodies

Product name

Biglycan Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with canine and mouse based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 2,000.

Reactivity

Human, Mouse

Host

Goat

Isotype

IgG

Vial size

100 μg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Biglycan using Biglycan Polyclonal Antibody (Product # PA5-18606) in paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing Golgi staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Biglycan using Biglycan Polyclonal Antibody (Product # PA5-18606) in paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing Golgi/ER staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).