MA1-19197

antibody from Invitrogen Antibodies

Targeting: LCK

Western blot (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19197

Provider

Invitrogen Antibodies

Product name

LCK Monoclonal Antibody (LCK-01)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen). This antibody will not cross-react with mouse.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

LCK-01

Vial size

100 μg

Concentration

1 mg/mL

Storage

4°C, do not freeze

References

Submitted references

The phosphoproteome of human Jurkat T cell clones upon costimulation with anti-CD3/anti-CD28 antibodies.

Nguyen TD, Carrascal M, Vidal-Cortes O, Gallardo O, Casas V, Gay M, Phan VC, Abian J
Journal of proteomics 2016 Jan 10;131:190-198

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LCK (LCK-01) purified Monoclonal antibody (Product # MA1-19197) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Separation of human CD3 positive LCK positive human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LCK (LCK-01) purified Monoclonal antibody (Product # MA1-19197) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LCK (LCK-01) purified Monoclonal antibody (Product # MA1-19197) (concentration in sample 9 µg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ using a dilution of 20 µL reagent/100 µL of peripheral whole blood.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LCK (LCK-01) purified Monoclonal antibody (Product # MA1-19197) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Separation of human CD3 positive LCK positive human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LCK (LCK-01) purified Monoclonal antibody (Product # MA1-19197) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LCK (LCK-01) purified Monoclonal antibody (Product # MA1-19197) (concentration in sample 9 µg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ using a dilution of 20 µL reagent/100 µL of peripheral whole blood.