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LearnPA5-28741
antibody from Invitrogen Antibodies
Targeting: CXCR3
CD183, CKR-L2, CMKAR3, GPR9, IP10-R, MigR
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-28741 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: K562, NCI-H929. Predicted reactivity: Rhesus Monkey (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1.
Jung O, Beauvais DM, Adams KM, Rapraeger AC
Journal of cell science 2019 Oct 28;132(20)
Journal of cell science 2019 Oct 28;132(20)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CXCR3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXCR3 Polyclonal Antibody (Product # PA5-28741) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR3 Polyclonal Antibody detects CXCR3 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR3 Polyclonal Antibody (Product # PA5-28741) diluted at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR3 Polyclonal Antibody detects CXCR3 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CXCR3 Polyclonal Antibody (Product # PA5-28741) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR3 Polyclonal Antibody detects CXCR3 protein at cytoplasm and membrane in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CXCR3 Polyclonal Antibody (Product # PA5-28741) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
