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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-27119 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FZD9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: U87-MG. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.77 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Frizzled 9 using 30 µg of U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Frizzled 9 polyclonal antibody (Product # PA5-27119) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FZD9 Polyclonal Antibody (Product # PA5-27119). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FZD9 Polyclonal Antibody (Product # PA5-27119) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FZD9 was performed in paraffin-embedded rat duodenum tissue using FZD9 Polyclonal Antibody (Product # PA5-27119) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U374 xenograft, using Frizzled 9 (Product # PA5-27119) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
