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LearnPA5-13263
antibody from Invitrogen Antibodies
Targeting: MTOR
FLJ44809, FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-13263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- mTOR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas.
Picot S, Faury N, Arzul I, Chollet B, Renault T, Morga B
Autophagy 2020 Nov;16(11):2017-2035
Autophagy 2020 Nov;16(11):2017-2035
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a mTOR polyclonal antibody (Product # PA5-13263) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of mTOR in HeLa cells. Samples were incubated in mTOR polyclonal antibody (Product # PA5-13263) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of mTOR in formalin-fixed and paraffin-embedded human testis tissue. Samples were incubated with mTOR polyclonal antibody (Product # PA5-13263) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7. Immunohistochemical detection of BECN1, MTOR, SQSTM1 and MAP1LC3A in several tissues of the Pacific oyster, Crassostrea gigas . (A) Mantle with connective tissue, nerf and muscle, (B) gonad, (C) gills, (D) digestive tubules, (E) muscle, (F) digestive gland epithelium, (G) mantle with connective tissue, (H) nerf in the mantle connective tissue, (I) gills, (J) digestive tubules, (K) mantle with connective tissue, (L) muscle, (M) digestive gland epithelium and connective tissue, (N) digestive tubule, (O) mantle with connective tissue, nerf and muscle, (P) gonad, (Q) gills, (R) digestive tubules, (S) mantle with nerf and muscle, (T) gonad, (U) gills and (V) digestive tubule. Scale bar: 20 um
