PA5-25788

antibody from Invitrogen Antibodies

Targeting: CUX1 CASP, CDP, CDP/Cut, CDP/Cux, CDP1, Clox, CUT, CUTL1, CUX, Cux/CDP, GOLIM6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-25788

Provider

Invitrogen Antibodies

Product name

CUX1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 μL

Concentration

1.8 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CUX1 in various lysates. Samples were incubated with CUX1 polyclonal antibody (Product # PA5-25788) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:15,000. Lysates/proteins: 20 µg per lane. Lane 1: SH-SY5Y whole cell lysate; Lane 2: SK-BR-3 whole cell lysate; Lane 3: NIH/3T3 whole cell lysate. Observed band size: 200 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CUX1 in various lysates. Samples were incubated with CUX1 polyclonal antibody (Product # PA5-25788) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: 293 whole cell lysate; Lane 2: K562 whole cell lysate. Predicted band size: 164 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CUX1 in various lysates. Samples were incubated with CUX1 polyclonal antibody (Product # PA5-25788) using a dilution of 1:2,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: MCF-7 whole cell lysate; Lane 2: MOLT-4 whole cell lysate; Lane 3: SH-SY5Y whole cell lysate. Predicted band size: 164 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using a CUX1 polyclonal antibody (Product # PA5-25788). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a CUX1 polyclonal antibody (Product # PA5-25788) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of CUX1 in MCF-7 cells. Samples were incubated with CUX1 polyclonal antibody (Product # PA5-25788) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing nucleus and weak cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1:100 dilution (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CUX1 in paraformaldehyde-fixed, paraffin-embedded human kidney tissue sections. Samples were incubated with CUX1 polyclonal antibody (Product # PA5-25788) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CUX1 in paraformaldehyde-fixed, paraffin-embedded human lymph node tissue sections. Samples were incubated with CUX1 polyclonal antibody (Product # PA5-25788) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CUX1 in paraformaldehyde-fixed, paraffin-embedded human kidney tissue sections. Samples were incubated with CUX1 polyclonal antibody (Product # PA5-25788) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of CUX1 in paraformaldehyde-fixed, paraffin-embedded human lymph node tissue sections. Samples were incubated with CUX1 polyclonal antibody (Product # PA5-25788) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).